Binding of nucleotide spin probes to AMV reverse transcriptase and metal ions
نویسندگان
چکیده
منابع مشابه
Nucleotide Reverse Transcriptase Inhibitors
4 Optimal HIV-1 suppression often is difficult to achieve and maintain with currently available antiretroviral drugs, and viral resistance eventually emerges to many drugs. Chronic antiretroviral therapy is also associated with complications such as body fat redistribution, hypertriglyceridemia and hypercholesterolemia, abnormal glucose metabolism, lactic acidosis, pancreatitis, avascular necro...
متن کاملUse of radiotracer probes to study the binding of metal ions to wool powders
The objective of the project is to examine the potential use of wool as an industrial and/or laboratory adsorbent. This will be carried out by examining the metal sorption and desorption behaviour of a series of wool powders using radiotracer probes. Wool powders will be prepared using various milling techniques, and their metal-binding properties investigated using radiotracers such as Co, Cu ...
متن کاملTargeted mutagenesis in vitro: lac repressor mutations generated using AMV reverse transcriptase and dBrUTP.
We have cloned the gene for the lac operon repressor (lacI) of Escherichia coli into the M13 related phage f1. Mutagenesis of the lacI gene was performed in vitro by filling dsDNA molecules gapped over the lacI gene with Avian Myeloblastosis Virus (AMV) reverse transcriptase. LacI mutants are found at a frequency of 1 in 10(4) using a genetic screen in vivo. For two-thirds of the 60 mutants, le...
متن کاملFunctional and structural dynamics of hepadnavirus reverse transcriptase during protein-primed initiation of reverse transcription: effects of metal ions.
Reverse transcription in hepadnaviruses is primed by the viral reverse transcriptase (RT) (protein priming) and requires the interaction between the RT and a specific viral RNA template termed epsilon. Protein priming is resistant to a number of RT inhibitors that can block subsequent viral DNA elongation and likely requires a distinct "priming" conformation. Furthermore, protein priming may co...
متن کاملTemperature-dependent template switching during in vitro cDNA synthesis by the AMV-reverse transcriptase.
Reverse transcriptase template switching has been invoked to explain several aspects of retroviral replication and recombination, and has been reported in vitro for the Moloney murine leukemia virus (M-MuLV) reverse transcriptase. During in vitro cDNA synthesis, the avian myeloblastosis virus (AMV) reverse transcriptase can switch from one template to another in a homology-dependent and tempera...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1982
ISSN: 0014-5793
DOI: 10.1016/0014-5793(82)80847-8